产品详情
  • 产品名称:重组马白介素-2 Cys141Ser蛋白

  • 产品型号:rEqIL-2 Cys141Ser
  • 产品厂商:KALANG
  • 产品文档:
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简单介绍:
重组马白介素-2 Cys141Ser蛋白与其它公司提供的重组蛋白不同,rEqIL-2 Cys141Ser蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组马白介素-2 Cys141Ser蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍:

重组马白介素-2 Cys141Ser蛋白

Species Equine
Accession P37997
GeneID 100034204
Source Escherichia coli.
Molecular Weight 重组马白介素-2 Cys141Ser蛋白Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
Quantity 2µg/10µg/1000µg
AA Sequence APTSSSKRET QQQLKQLQMD LKLLLEGVNN NKNPKLSKML TFKINMPKKA TELKHLQCLE EELKPLEEML KNFLSKDIKE LMSNINVTVL GLKGSETRFT CEYDDETGTI VEFLNKWITF SQSIFSTMT
Purity 重组马白介素-2 Cys141Ser蛋白> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 重组马白介素-2 Cys141Ser蛋白Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 6.5.
Endotoxin Less than 0.1 EU/µg of rEqIL-2 Cys141Ser as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组马白介素-2 Cys141Ser蛋白
SDS-PAGE

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