产品详情
简单介绍：
重组犬肿瘤坏死因子-α/ TNFSF2蛋白与其它公司提供的重组蛋白不同，rCaTNF-α/TNFSF2蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组犬肿瘤坏死因子-α/ TNFSF2蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组犬肿瘤坏死因子-α/ TNFSF2蛋白
| Species | Canine |
| Accession | P51742 |
| GeneID | 403922 |
| Source | Escherichia coli. |
| Molecular Weight | 重组犬肿瘤坏死因子-α/ TNFSF2蛋白Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | VKSSSRTPSD KPVAHVVANP EAEGQLQWLS RRANALLANG VELTDNQLIV PSDGLYLIYS QVLFKGQGCP STHVLLTHTI SRFAVSYQTK VNLLSAIKSP CQRETPEGTE AKPWYEPIYL GGVFQLEKGD RLSAEINLPN YLDFAESGQV YFGIIAL |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组犬肿瘤坏死因子-α/ TNFSF2蛋白Fully biologically active when compared to standard. The ED50as determined by a cytotoxicity assay using murine L929 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106IU/mg in the presence of actinomycin D. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rCaTNF-α as determined by LAL method. |
| Reconstitution | 重组犬肿瘤坏死因子-α/ TNFSF2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组犬肿瘤坏死因子-α/ TNFSF2蛋白 | |
| SDS-PAGE |  |
