产品详情
简单介绍：
重组大鼠心肌营养素-1蛋白与其它公司提供的重组蛋白不同，rRtCT-1蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠心肌营养素-1蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组大鼠心肌营养素-1蛋白
| Species | Rat |
| Accession | Q63086 |
| GeneID | 29201 |
| Source | Escherichia coli. |
| Molecular Weight | 重组大鼠心肌营养素-1蛋白Approximately 21.4 kDa, a single non-glycosylated polypeptide chain containing 203 amino acids. |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组大鼠心肌营养素-1蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1 % TFA. |
| Endotoxin | Less than 1 EU/μg of rRtCT-1 as determined by LAL method. |
| Reconstitution | 重组大鼠心肌营养素-1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组大鼠心肌营养素-1蛋白 | |
| SDS-PAGE |  |
| Reference | 1. Pennica D, Wood WI, Chien KR. 1996. Cytokine Growth Factor Rev. 7:81-91. 2. Robledo O, Fourcin M, Chevalier S, et al. 1997. J Biol Chem. 272:4855-63. 3. Gerhard DS, Wagner L, Feingold EA, et al. 2004. Genome Res. 14:2121-7. |
| Background | Cardiotrophin1 (CT1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the ***** heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1. |
