产品详情
简单介绍：
重组大鼠白细胞介素-6蛋白与其它公司提供的重组蛋白不同，rRtIL-6蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠白细胞介素-6蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组大鼠白细胞介素-6蛋白
| Species | Rat |
| Accession | P20607 |
| GeneID | 24498 |
| Source | Escherichia coli. |
| Molecular Weight | 重组大鼠白细胞介素-6蛋白Approximately 21.7 kDa, a single, non-glycosylated polypeptide chain containing 187 amino acids |
| Quantity | 2µg/10µg/1000µg |
| AA Sequence | FPTSQVRRGD FTEDTTHNRP VYTTSQVGGL ITYVLREILE MRKELCNGNS DCMNSDDALS ENNLKLPEIQ RNDGCFQTGY NQEICLLKIC SGLLEFRFYL EFVKNNLQDN KKDKARVIQS NTETLVHIFK QEIKDSYKIV LPTPTSNALL MEKLESQKEW LRTKTIQLIL KALEEFLKVT MRSTRQT |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组大鼠白细胞介素-6蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rRtIL-6 as determined by LAL method. |
| Reconstitution | 重组大鼠白细胞介素-6蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HCl to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组大鼠白细胞介素-6蛋白 | |
| Reference | 1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8. 2. van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44. 3. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2. 4. Northemann W, Braciak TA, Hattori M, et al. 1989. J Biol Chem. 264:16072-82. 5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314. 6. Van Snick J, Cayphas S, Szikora JP, et al. 1988. Eur J Immunol. 18:193-7. |
| Background | Interleukin-6 (IL-6) is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. IL-16 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The rat IL-6 is a single non-glycosylated polypeptide chain containing 187 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal-transducing component gp130 (also called CD130). The rat IL-6 shares 40 % and 85 % aa sequence identity with human and mouse IL-6 and it is equally active on human and mouse cells. |
