产品详情
简单介绍：
重组大鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白与其它公司提供的重组蛋白不同，rRtMIP-1β/CCL4蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组大鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍：
重组大鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白
| Synonyms | Small-inducible Cytokine A4 |
| Species | Rat |
| Accession | P50230 |
| GeneID | 116637 |
| Source | Escherichia coli. |
| Molecular Weight | 重组大鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids. |
| Quantity | 5µg/20µg/1000µg |
| AA Sequence | APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重组大鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重组大鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4, 3 % trehalose. |
| Endotoxin | Less than 1 EU/µg of rRtMIP-1β/CCL4 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重组大鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白 | |
| Reference | 1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9. 2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31. 3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94. |
| Background | Chemokine (C-C motif) ligand 4 encoded by the CCL4 gene, also known as macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant rat CCL4 contains 69 amino acids and it shares 80% and 86% a.a. sequence identity with human and murine CCL4. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells. |
