产品详情
  • 产品名称:重组鼠心肌营养素1蛋白

  • 产品型号:rMuCT-1
  • 产品厂商:KALANG
  • 产品文档:
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简单介绍:
重组鼠心肌营养素1蛋白与其它公司提供的重组蛋白不同,rMuCT-1蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠心肌营养素1蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
详情介绍:

重组鼠心肌营养素1蛋白

Species Murine
Accession Q60753
GeneID 13019
Source Escherichia coli.
Molecular Weight 重组鼠心肌营养素1蛋白Approximately 21.5 kDa, a single non-glycosylated polypeptide chain containing 203 amino acids.
Quantity 2µg/10µg/1000µg
AA Sequence MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC GLYGEWVSRT EGDLGQLVPG GVA
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity 重组鼠心肌营养素1蛋白Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.
Endotoxin Less than 1 EU/µg of rMuCT-1 as determined by LAL method.
Reconstitution 重组鼠心肌营养素1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组鼠心肌营养素1蛋白
SDS-PAGE
Reference 1. Pennica D, Wood WI, Chien KR. 1996. Cytokine Growth Factor Rev. 7:81-91.
2. Robledo O, Fourcin M, Chevalier S, et al. 1997. J Biol Chem. 272:4855-63.
3. Gerhard DS, Wagner L, Feingold EA, et al. 2004. Genome Res. 14:2121-7.
Background Cardiotrophin1 (CT1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the ***** heart, skeletal muscle, ovary, colon, prostate and fetal lung. In addition, CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Murine CT1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide. Human and murine CT1 share 80 % a.a. sequence identity and exhibit cross-species activity.

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